Previous articleMagee’s bow out of French Open Super SeriesNext articleAlmost €24 million paid out to community organisations in Donegal admin 365 additional cases of Covid-19 in Republic By admin – October 23, 2015 The twin sister of Mary Boyle who went missing in 1977 will visit Westminster Tuesday next for a series of meetings led by MP Jeffrey Donaldson to address Garda failure and cross-border crime.A number of families have been invited to London to discuss the issues.It’s believed Ann Doherty will also reiterate her claim to know the identity of Mary Boyles alleged killer during this meeting.Mary Boyle went missing from her grandparents farm at Cashelard near Ballyshannon on the 18th of March 1977.Journalist Gemma O’Doherty says this will put the case on an international platform, in the hope that justice will be achieved:Audio Playerhttp://www.highlandradio.com/wp-content/uploads/2015/10/gemraw.mp300:0000:0000:00Use Up/Down Arrow keys to increase or decrease volume. Main Evening News, Sport and Obituaries Tuesday May 25th Further drop in people receiving PUP in Donegal Man arrested on suspicion of drugs and criminal property offences in Derry Pinterest Twitter Facebook 75 positive cases of Covid confirmed in North WhatsApp Twitter Mary Boyle’s sister to meet with MPs in Westminster WhatsApp Gardai continue to investigate Kilmacrennan fire Google+ Google+ RELATED ARTICLESMORE FROM AUTHOR Homepage BannerNews Facebook Pinterest
Home » COVID-19 support » Official: branches will be able to stay open during lockdown previous nextCOVID-19 newsOfficial: branches will be able to stay open during lockdownThe Government has announced that estate and letting agents can open their branches during the forthcoming lockdown.The Negotiator3rd November 2020026,798 Views Uncertainty over whether estate and letting agencies will be classified as essential services under Thursday’s lockdown rules, has been clarified.In a statement to the industry, the Government says: “Buying, selling and renting a home can continue, in a COVID-secure way, as it has in recent months. Estate and letting agents can operate, show homes and sales suites can remain open and property viewings, mortgage valuations and surveys can take place.”Subject to approval, the regulations specifically state that activities relating to the sector are allowed to continue under exceptions to leaving home to undertake any of the following activities in connection with the purchase, sale, letting or rental of a residential property:visiting estate or letting agents, developer sales offices or show homes;viewing residential properties to look for a property to buy or to rent;preparing a residential property to move in;moving house;visiting a residential property to undertake any activities required for the rental orsale of that property.Commenting for Propertymark, Timothy Douglas (left), Policy and Communications Manager said: “Following the laying of regulations for national lockdown measures in England we are very pleased that the hard work of our members and the importance of the property sector has been recognised with exceptions in the rules for home moves as well as visits to branches and allowing agents to visit property to undertake any required activities.“This is of course, subject to approval by Parliament. However, at this stage all the indications are that buying, selling and renting a home will continue and must be done in a COVID secure way and in accordance with the guidance.”Read more about the latest lockdown.estate agent lockdown Timothy Douglas lockdown NAEA Property November 3, 2020Grant LeonardWhat’s your opinion? Cancel replyYou must be logged in to post a comment.Please note: This is a site for professional discussion. Comments will carry your full name and company.This site uses Akismet to reduce spam. Learn how your comment data is processed.Related articles BREAKING: Evictions paperwork must now include ‘breathing space’ scheme details30th April 2021 City dwellers most satisfied with where they live30th April 2021 Hong Kong remains most expensive city to rent with London in 4th place30th April 2021
The AGO confirmed on Monday that Pinangki was still a prosecutor and a member of the Indonesian Prosecutors Association (PJI) despite her suspect status, and thus, she would receive legal assistance from lawyers appointed by the association, kompas.com reported.Antigraft watchdog Indonesia Corruption Watch (ICW) researcher Kurnia Ramadhana said Pinangki’s alleged meeting with Djoko, then an AGO fugitive, had tarnished the image of the institution itself and, therefore, she “does not deserve legal assistance” from the PJI.The AGO itself has declared Pinangki guilty of committing an ethics violation by going on nine international trips to Singapore and Malaysia in 2019 without permission, during which she allegedly met with Djoko.Given the alleged violations, Kurnia said that providing Pinangki with legal assistance would contradict the PJI’s rules of association and internal bylaws (AD/ART). Read also: Napoleon Bonaparte among two police generals named graft suspects in Djoko Tjandra case”The AD/ART stipulates that PJI aims to defend and assist its members who face legal issues related to their professional duties. However, what the prosecutor Pinangki did was not related to her duties and profession, because the meeting was held without her superior’s approval and it was with the fugitive,” Kurnia said.“There is a term ‘the blue wall of silence’ in the United States when law enforcers stay silent or protect their colleagues who are involved in illegal practices. We don’t want that to happen.”Meanwhile, Indonesian Anticorruption Community (MAKI) coordinator Boyamin Saiman said the prosecutor’s organization should not provide legal assistance to Pinangki because of ethical reasons, arguing that she got entangled in the case not because of her duties but because she had violated her duties as prosecutor.”Pinangki should find herself a lawyer instead of getting the PJI assistance,” he said.Boyamin, however, conceded that assistance from the PJI was not legally problematic as the association did not represent the AGO, but it was an organization in which Pinangki was a member.Pinangki has been removed from her post as an official in the planning bureau under the AGO’s assistant attorney general.Djoko, a convict in the high-profile Bank Bali corruption case, was arrested in Malaysia on July 30. He had been at large for 11 years after fleeing Indonesia a day before the Supreme Court sentenced him to two years in prison and ordered him to pay Rp 546 billion (US$54 million) in restitution.Topics : Anticorruption activists have expressed their disapproval of the legal assistance made available by a prosecutors association for Pinangki Sirna Malasari, a prosecutor who was arrested for allegedly accepting bribes in connection with graft convict Djoko Tjandra. Pinangki was named a bribery suspect last week following an investigation by the Attorney General’s Office (AGO) into her alleged meetings with Djoko when the latter was still on the run in neighboring countries last year. She was suspected to have accepted about US$500,000 in bribes, though the AGO did not specify who she got it from.
Behind the scenes it takes a lot of hard work. From his diet, to exercise routine to sleep and rest, the forward leads the most disciplined of lifestyles. He can eat up to six meals a day, with fish a big part of his diet. Once tucked up, Ronaldo will ensure he has eight hours of sleep each night. That’s in addition to FIVE naps he will take during the day. The gym and intense exercise are also a huge part of Ronaldo’s life – as shown by his incredible muscle definition and speedy recovery from injury. Manchester United star Jesse Lingard recently described Ronaldo as the Michael Jordan of football. He told Sky Sports: “Everything he has achieved in his career. He has been at plenty of clubs and won many trophies. “I believe he is a real icon of football, the Michael Jordan of football.” FacebookTwitterWhatsAppEmail分享 Loading… World football legend,Edson Arantes do Nascimento, popularly known as Pele, has described Juve striker, Cristiano Ronaldo as a real icon of football and modern athlete, who shows everyone that success always comes for those who are committed and love what they do. The FIFA Ambassador lauded Ronaldo for his incredible feat after winning the second title for Juventus. The 79-year-old wrote on Instagram: “Cristiano Ronaldo is the modern athlete. I want to pay tribute to the incredible Douglas Costa , Alex Sandro and Danilo. Congratulations on the championship, Juventus!” The Portuguese five-time Ballon d’Or winner won his second Serie A title in just two seasons since joining Italian giants Juventus. The 35-year-old extended his tally to 31 league goals during the Old Lady’s 2-0 win over Sampdoria that confirmed top spot. And Ronaldo came in for particular praise from Pele, who has previously named the ex-Real Madrid superstar as the best in the world. Despite entering the twilight years of his career Ronaldo has become the fastest man to net 50 Serie A goals.Advertisement Promoted ContentThe Highest Paid Football Players In The World10 Of The Most Powerful Women Across The GlobeThe Very Last Bitcoin Will Be Mined Around 2140. Read MoreSuperhero Castings That People Hated But Were AmazingEver Thought Of Sleeping Next To Celebs? This Guy Will Show You10 Risky Jobs Some Women Do8 Things To Expect If An Asteroid Hits Our PlanetThese TV Characters Proved That Any 2 People Can Bury The HatchetBest & Worst Celebrity Endorsed Games Ever Made7 Ways To Understand Your Girlfriend Better2020 Tattoo Trends: Here’s What You’ll See This YearWhy Go Veg? 7 Reasons To Do This
Vancouver needs to stick to their game. While their compete level wasn’t perfect at all times tonight, the Canucks have been the better team at even strength in this series. If they can clean up the special teams, score occasionally on the power play, and not give up copious amounts of shorthanded goals, they will be successful. The Canucks put a much better effort on the ice tonight, but special teams were their Achilles heel, to the point that I think it’s more appropriate to refer to them as “Special Needs Teams”. The power play, struggling as far back as mid-December, was again 0 for 5. While the power play generated 10 shots on net, at times it was ridiculously poor. The Canucks had trouble gaining the Kings’ zone on multiple occasions, and continually made bad passes and other mistakes which prevented them from generating a lot of scoring chances.Not only did the power play fail to generate offense, it allowed the two shorthanded goals. Alex Edler, who is playing his worst hockey of the season at exactly the wrong time, gave the puck to Anze Kopitar in the defensive zone, a gaffe that led to the Kings’ first goal. Dan Hamhuis, one of the Canucks’ steadiest defensemen, stumbled at the point which allowed Brown to speed past him, and score on a breakaway to put the Kings in front for good early in the second period. Truly, it’s at the point that the Canucks should not ice their fifth skater, it’s better if they play even-up if the other team takes a penalty.The Kings’ power play added a goal of its own midway through the third period, which effectively iced the game.So far in this series, the Kings have scored seven non-empty net goals. Of those, they have scored three on the power play in 12 opportunities, and have two shorthanded goals in 10 Canuck power plays. On the flip side, the Canucks are 0 for 10 on the power play. At even strength, the Canucks have badly outshot L.A., and have actually outscored them 4-2. GAME OBSERVATIONS After watching tonight’s game, one really wonders what is going on with this team. What happened to the team that we iced last season in the playoffs? These guys just appear disinterested and for the second night in a row, the Canucks were outworked too often, losing puck battles, and generally not moving their legs and losing races to the puck. In the playoffs, desire is what is required, as a team’s will is more important than its skill. The Kings have appeared the hungrier team thus far in the series and that is why they are ahead 2-0. Their forecheck has been right on top of our D-men and they’ve been turning the puck over. It’s ugly to watch, and the Canucks need to work harder and think more quickly. Alex Edler. In my mind, coach Alain Vigneault needs to seriously consider making him a healthy scratch and having him watch from the press box. Saying that is something, Edler was an all-star this year and had a great first half. His play in the second half has been just this side of horrible and tonight, it was even worse. Brutal giveaway on the first goal, and another on the third goal when he was unable to clear the puck. He just doesn’t look right. Roberto Luongo was good in net for the Canucks tonight. That said, his numbers in the first two games are now as follows: GAA – 3.62, SV% – .891. Those numbers are not good enough to win. I’m certainly NOT “blaming” Luongo, his performance tonight was not like we have seen in his meltdowns – this is NOT his fault. However, the goals tonight were all stoppable, if he were able to come up with a big save. He wasn’t his best tonight. Two games is a small sample size, and I get that, but at some point the numbers don’t lie. We saw a classic “Luongo on his belly” on the Brown breakaway, and a bad “leak through” goal in the third. Leigh Ramsden lives in Vancouver and is an avid Canucks fan, having been a partial season ticket holder for over 10 years. He’s old enough to have witnessed all three Stanley Cup losses, as such, his prime goal is to remove those scars by seeing a Cup brought to Vancouver. Leigh is Fighting For Stanley’s (www.fightingforstanley.ca/vancouver) west coast correspondent, and will also blog after all Canuck games for The Nelson Daily.The Vancouver Canucks entered game 2 against the L.A. Kings knowing that another effort like they had in game one wouldn’t be good enough. On Friday night at Rogers Arena, the Canucks played better, but special teams absolutely killed them as they dropped a 4-2 decision to the L.A. Kings, as they went down 2-0 in the series. The Canucks are now facing a monumental uphill battle, as they will have to win four games out of the next five with three of those coming on the road.Dustin Brown led the Kings offensively and was a deserving recipient of the game’s first star, as he scored twice shorthanded and added another assist. No other Kings tallied more than one point. Generally, the Kopitar-Brown-Williams line has been a beast in the first two games. The Richards-Carter-King line, dominant in game one, pretty much disappeared tonight, finishing minus-6 while recording no points.Overall, the Canucks were pretty dominant 5 on 5 against the Kings. The Canucks ended up outshooting L.A. 48-26 in the game, and 38-26 in the second and third periods. While everyone is ruing the Canucks’ inability to score, the team built itself to win tight, low scoring games and started playing a more conservative style coming down the stretch. Similar to last year’s finals, I’m not pinning these losses on an inability to score – rather, the Canucks are not keeping the puck out of the net. The team needs to score more, for sure – but as they were built and the style they have been playing, four goals against is too many if they want to win. I lambasted Ryan Kesler in this space after game 1 – and tonight, he was way better. He was the Canucks’ best player, worked hard all night, and was the only forward who consistently made things happen in the Kings’ zone. Also, he didn’t dive, chirp the referees, or the other team. It was an infinitely better performance from him and tonight he gave the team what it needed. More games like this, and the puck will start going in for him and his linemates. What is happening to the Canucks is not reflecting well on the coaching staff. If the Canucks lose this series, there are a lot of questions that will be asked about Vigneault and his ability to motivate this group of players. In my opinion, only a Stanley Cup win should save his job; but more realistically, if the Canucks lose in the first round, I think it’s virtually assured that he’ll be heading up the Montreal Canadiens next year. PARTING SHOTSBroadcast Observation of the Night: Bruce Boudreau has been a nice addition to the Hockey Night in Canada broadcast. He obviously knows the game, and even though he’s employed in the league, he’s not afraid to speak his mind and tell it like he is. He’s a very affable sort of guy and I think he’s great, his analysis is sound, and the fact he’s a current NHL coach lends him an aura of respectability. He’s a great addition to their coverage. Two things on the power play: 1) The neutral-zone drop pass – this hasn’t worked well for MONTHS. It worked well early in the season, but around Christmas (when the power play went into the tank), teams were figuring it out and giving the Canucks fits in the neutral zone. They need to just get rid of it – it led directly to the Kings first goal. 2) One thing I’ve noticed is that the teams having success killing our penalties are being extremely aggressive on the puck while they’re killing the penalty, and our guys just aren’t moving the puck fast enough. I’m not a coach in the NHL, and it’s obvious to me – I don’t understand why it’s not obvious to the team’s coaching staff. Every team is doing the same thing, and it’s working. Newell Brown – crunch time – you need to earn your paycheque. Make some adjustments and get it working. TAKING STOCK OF WHERE WE’RE AT It’s too early to write the Canucks off. Just last year, the Boston Bruins lost the first two games of the playoffs at home, and we all know how that turned out for them. In addition, Cam Charron at Canucks Army noted that back in 2001, the upstart Canucks jumped out to a 2-0 lead over the Presidents’ Trophy winning Detroit Red Wings, before Detroit found their game and steamrolled the Canucks in the next four games straight. Statistics show that a team going down 2-0 still has an approximate 25% chance of winning, so this series is far from over. In recent years, we’ve seen numerous series look over but then the momentum changes and the team that’s down comes back – consider the Blackhawks against the Canucks last year in the first round. Perhaps freshest in their minds will be the Stanley Cup Final – the Canucks won the first two games and Boston came back to win. It’s very easy to get discouraged after losing a couple games like this, but this mountain in front of the Canucks right now is not too big to climb. I predicted a seven game series here and I still think the Canucks are going to play better on the road and hopefully climb back into the series. At this point, they need to take things “one game at a time” – it’s the only way to go forward. Lots of chatter after the game tonight about who should start in net in game 3. Last year, in the first round after two consecutive losses, Vigneault started Schneider to mix things up. I am very confident that Schneider will be the starter for game 3. As noted, Luongo wasn’t bad tonight – but he wasn’t that great either. The team does seem to play better and tighter defensively in front of Schneider, and at this point, a change is needed. There were many warning signs of this type of performance as the team came down the stretch. However, at all times I had faith that the team, having learned lessons in playoff years past, knew what it would take to get the job done. At this point, I have to seriously question whether I was right. So far, it appears I wasn’t.
Montserrado County District #10 Rep. Yekeh Kolubah threatened expulsion from the House of Representatives for his utterances. – Advertisement – — Rep. Fallah disclosesTough talking and controversial District# 10 Representative Yekeh Kolubah has been threatened with expulsion from the House of Representatives if he does not , within three months, retract and apologize for remarks he allegedly made about unseating the government of President Weah.According to Montserrado County District#5 Representative Thomas Fallah if Rep. Kolubah does not desist from consistently and continuously bringing the House of Representatives into ‘disrepute’ with his ‘reckless and insulting comments’ he will either be suspended if he is lucky, or expelled based on Rule 40 of the House’s Rules and Procedures.Rep. Fallah said majority members of the House, at least 49 members, in accordance to the House’s Rules and Procedures, will either suspend or expel Rep. Kolubah for ‘bad conduct and undesirable acts.’“I want to use this time to urge my colleague and my nephew (that’s how we call one another in Lofa), Rep. Kolubah, to desist from raining insults and bringing the Honorable House of Representatives [into disrepute] and, if he does not desist and continues as of this (Tuesday) evening or tonight, if he is lucky he will be suspended and, when bad-lucky, he will be expelled,” he said.Rep. Fallah further urged Rep. Kolubah to limit his advocacy on policy issues, including the economy, health and other basic social issues, but not make unsubstantial issues and rain insults, which will cast a dark cloud on the Legislature and the government.But legal experts here, commenting on Representative Fallah’s remarks, have scoffed the idea of having Yekeh expelled from the body on account of statements he has allegedly made against the President. They maintain that threats to have Yekeh expelled if actualized could provoke a constitutional crises with implications for peace and stability in Liberia.They further noted that such threats are intended to institutionalize the criminalization of free speech and expression s guaranteed under Article 15 of the Constitution.Article 15 of the Constitution provides the following: a) Every person shall have the right to freedom of expression, being fully responsible for the abuse thereof. This right shall not be curtailed, restricted or enjoined by government save during an emergency declared in accordance with this Constitution.b) The right encompasses the right to hold opinions without interference and the right to knowledge. It includes freedom of speech and of the press, academic freedom to receive and impart knowledge and information and the right of libraries to make such knowledge available. It includes non-interference with the use of the mail, telephone and telegraph. It likewise includes the right to remain silent.c) In pursuance of this right, there shall be no limitation on the public right to be informed about the government and its functionaries.d) Access to state owned media shall not be denied because of any disagreement with or dislike of the ideas express. Denial of such access may be challenged in a court of competent jurisdiction.e) This freedom may be limited only by judicial action in proceedings grounded in defamation or invasion of the rights of privacy and publicity or in the commercial aspect of expression in deception, false advertising and copyright infringement.Moreover, according to a leading legal practitioner(name withheld) , Article 42 of the Constitution of Liberia provides that “No member of the Senate or House of Representatives shall be arrested, detained, prosecuted or tried as a result of opinions expressed or votes cast in the exercise of the functions of his office”.Article 42 further provides that “Members shall be privileged from arrest while attending, going to or returning from sessions of the Legislature, except for treason, felony or breach of the peace. All official acts done or performed and all statement made in the Chambers of the Legislature shall be privileged, and no Legislator shall be held accountable or punished therefor”.The legal practitioner questioned whether or not Yekeh is being threatened with contempt of the Legislature. And in case he is, according to the lawyer, Article 44 of the Constitution of Liberia specifically provides that “Contempt of the Legislature shall consist of actions which obstruct the legislative functions or which obstruct or impede members or officers of the Legislature in the discharge of their legislative duties and may be punished by the House concerned by reasonable sanctions after a hearing consistent with due process of law”.Article 44 further provides that “No sanctions shall extend beyond the session of the Legislature wherein it is imposed, and any sanction imposed shall conform to the provisions on Fundamental Rights laid down in the Constitution”.According to the lawyer the last part of Article 44 has to do with disputes between legislators and non-members which, according to the Constitution, shall not be entertained or heard in the Legislature if such disputes are properly cognizable before the courts. But more to that, according to the lawyer, under Article 20 of the Constitution Yekeh cannot be deprived of his privilege as a legislator “except as the outcome of a hearing judgment consistent with the provisions laid down in the Constitution and in accordance with due process of law”.The lawyer in closing said gone are the days when an elected member of the House could be expelled at the whims of the Executive under the cover of what was at the time referred to as a Joint Resolution (JR). He observed that during TWP rule a former legislator, at the time from Grand Gedeh County, Chea Cheapoo was expelled from the House because he had run afoul of the True Whig Party elites who dominated local politics, according to the lawyer.Meanwhile it remains to be seen whether Representative Fallah can indeed make good on his threats which may, more likely than not, prove untenable.Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window)
This week we are going to take a hop, skip and a jump across the road from Mount Errigal to another iconic Donegal landmark, the ‘Poisoned Glen’.Have you ever wondered how such a spectacular place could be cursed with such a cruel, cold and callous name? Well let’s see what’s lurking in the past…Toxic Moss & Balor na Suíle Neimhe (Balor of the Evil Eye)… Some say that the Poisoned Glen owes its name to the former presence of the highly toxic Irish Spurge Moss. It is rumoured that the plant poisoned the drinking water and although the Spurge Moss is now extinct here farmers are still advised to keep their animals away from the Glen’s waters.Another reason for the name comes from times past, when it was said, Balor King of Tory had a daughter more beautiful than any eye could behold. Such was her beautiful radiance, and such were the ethics of the time, he decided it was best to imprison her in a lonely isolated tower, so she would never come within sight of man. But word of her stunning looks spread far and wide. Subsequently many attempts were made on her lonely cell and it wasn’t long before one would be Romeo succeeded in breaking in and taking her away across the water.The enraged Balor followed her across the seas to Magheroarty and he stalked her captor across the mainland, killing him with a giant stone. One such stone stands in a prominent position about 600meters up the glen and is said to be the evil, or poisoned, eye of Balor.Lost in translation… The more generally accepted version of why the ‘glen’ has the name that it does, is based simply on a mistake made by an English cartographer, who made a mistake in the translation from Irish to English.The mistake arose due to the fact that the Irish word for poison, “neimhe” (pronounced niv-uh), differs in its spelling by only one letter (the letter ‘I’) from the word for Heaven, “neamh” (pronounced nyow-uh).The glen used to be called the ‘Heavenly Glen’ by local people, as it was to them like they imagined Heaven itself to be and standing in the glen you can’t help but marvel at nature’s wondrous forces.Dunlewy Church…A striking landmark of this area is the roofless, white marble and blue quartzite church which is located near the car park area at the entrance to the glen. The church was built by Jane Smith Russell, as a memorial to her husband, James Russell who died September 2nd 1848. James Russell, was the landlord of the Dunlewey estate, was laid to rest in a vault under the church floor. Over time the local congregation diminished and as the estate’s resources also did too, maintaining the up keep of the church was no longer sustainable. So in 1955 it was decided to remove the roof for safety reasons and various items such as the church furniture went to churches in Derry and Raphoe. The main church bell is now installed in Cashel Church of Ireland near Doe Castle.Fancy a walk?This is an accessible location, at only 80meters above sea level, it is low level and yet it feels really mountainous! There is a minor pathway to the South East side of the main river, that’s the left hand bank as you walk into the glen! The pathway is rough, so good sturdy footwear should be worn. You follow this pathway both into and out of the glen. Care should be taken after or during heavy rainfall, as rivers in the area can rise very quickly. You can enjoy the area on many levels, be it from the car park at the mouth of the glen or after venturing further up towards Balor’s eye. So all in all it’s a great place for the family, novice or serious hill walker a like, enjoy!Map required: OSI Sheet Number 1, Scale 1:50,000Local attractions:www.dunleweycentre.comwww.glenveaghnationalpark.ieA great place to stay locally is the Errigal Youth Hostel, check them out on Facebook!http://www.facebook.com/errigal.hostelFor some excellent photos of the area check out:http://www.facebook.com/pages/Karl-Harold-Photography/201685779861848THE GREAT DONEGAL OUTDOORS – THE POISON GLEN by Brendan Whelan was last modified: May 18th, 2012 by StephenShare this:Click to share on Facebook (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Click to share on Reddit (Opens in new window)Click to share on Pocket (Opens in new window)Click to share on Telegram (Opens in new window)Click to share on WhatsApp (Opens in new window)Click to share on Skype (Opens in new window)Click to print (Opens in new window)Tags:bren whelanPoison GlenThe Great Donegal Outdoors
The California State Chamber of Commerce and the Grand National Rodeo Horse and Stock Show recently announced the selection of Jerry Hemsted as the 2016 Livestock Man of the year.Hemsted will accept the prestigious award at the Cow Palace in San Francisco on Saturday evening, Oct. 15.Hemsted was born into the cattle industry. He demonstrated his first love of the industry with the purchase of a heifer named “Domino Princess the 44th” at the age of 10 in Millville. He partnered with his dad …
ALAMEDA — We’ve seen a lot of growth in the second year of the union between Derek Carr and Jon Gruden.Now comes the next step, and in the case of Carr it’s more of a leap.Arrowhead Stadium is the most hostile of environments for Carr. His record there is 0-5, his stats are awful and not once has he passed the eye test of being a solid, functional NFL quarterback.When the Raiders (6-5) visit the Kansas City Chiefs (7-4) Sunday afternoon, Carr might not win, but he needs to keep the Raiders …
ATP synthase, the miniature rotary motor that powers our cells, has been a subject of great interest since the elucidation of its rotary function won three scientists a Nobel prize in 1997. As an example of a precision-crafted, true electric rotary motor in living systems (another being the larger bacterial flagellum), it also provides a classic case study in intelligent design vs. evolution. It has been the subject of frequent updates in these pages (start at 02/13/2004 and work backwards). Now, another discovery about this ATP-synthesizing engine has revealed a deeper level of fine tuning. Japanese scientists publishing in PNAS1 found a precision coupling between two components that was unexpected, yet apparently essential. For review, recall that ATP synthase has two functional domains, named F0 and F1. The F1 part that actually synthesizes ATP from ADP + P is now fairly well understood. It is composed of three pairs of lobes that spring-load ATP with every 120o turn of the camshaft, each pair of lobes either loading, catalyzing or ejecting an ATP molecule. The F0 domain, however, has been harder to study. Scientists knew it looks like a carousel of identical proteins, labeled c subunits. Linked to it is a camshaft, named the gamma subunit, that drives the synthesis of ATP in F1. Scientists knew the F0 carousel runs on protons delivered by a gumball-like mechanism named the a subunit (see 12/22/2003 headline). But up till now, they were not sure how many c subunits comprised the carousel – or even if the number mattered. Some studies had hinted that the F0 motor contained anywhere from 8 to 13 c subunits, depending on the species. Now, the team of Mitome et al. found the answer: it is 10, and it must be 10 and only 10. Other numbers don’t work. That’s strange. It means that F0 needs 10 protons per revolution, but F1 produces 3 ATP per revolution. The ratio 10:3 is not an integer. How can that be? The scientists arrived at the number 10 by customizing F0 rings with fixed numbers of c subunits, 2 through 14. Then they linked them to the F1 domains and watched how much ATP was synthesized. Results were obtained for only c=2, 5, and 10, which is interesting, considering that 2 and 5 are factors of 10. The c=2 and c=5 cases produced a little ATP, and c=10 produced the maximum. All the other numbers produced none. The team deduced, therefore, that 10 (or one of its factors) is essential to match the proton-loading mechanism of the a subunit. The scientists also measured the proton flow through their custom carousels when disengaged from F1 and found, again, that 10 was the only number that worked. Without 10 c subunits, no protons flowed. Divide a circle of 360o by 10, and you get a 36o angle per c subunit during a complete revolution of the F0 motor. The F1 domain, by contrast, produces ATP for each 120o turn, or 3 ATP per complete revolution. The scientists seemed surprised that the proton-ATP ratio, “one of the most important parameters in bioenergetics,” is not an integer. It’s as if three protons are sufficient to generate an ATP sometimes and four other times, because one cannot have a third of a proton. Wouldn’t it be more logical if the number of c subunits was a multiple of three, say 6, 9, or 12? With c=9, for instance, the camshaft angle would regularly line up with the F1 lobes every 3 protons, yielding one ATP every time, nice and neat. The fact that it does not means that the coupling between F0 and F1 is not strict, as with toothed gears, but “permissive” – as if the two domains rotate according to their own structural needs, and are coupled together by a adaptor mechanism that has some degree of freedom to either twist or slip. The scientists ruled out slippage. They knew that the camshaft can only produce an ATP in the F1 domain when it is lined up perfectly at the 120o steps. Instead, they found that there is enough elastic flexibility in the camshaft to permit twist up to 40o during its rotation. This flexibility allows the two domains to work separately, each according to its optimum configuration, with the twisting camshaft able to rock back and forth a little to give the F1 lobes time to complete their work. In scientific lingo, “The flexibility of gamma allows both the F0-gamma and F1-gamma interfaces at the free-energy minima to stay in conformations adequate for the proton transport in F0 and the catalysis in F1 despite the step-size mismatch, providing sufficient time for those events to take place.” One more thing. There isn’t much tolerance for error in this system. The team found that a single point mutation at a spot named E56 in the c subunit was enough to quench all proton flow and all ATP synthesis: “This result provides evidence that each of all 10 E56 in the c-ring is indispensable.” Also, the quantity of 10 subunits in the c-ring is critical, because 8, 9, 11, 12 and other numbers did not fit the gumball proton-delivery system of the a subunit: “Thus, the proton transport through F0 requires very strict arrangement of contact surface between F0-a and F0-c in the F0 assembly and even a rotary displacement as tiny as 3.3o (360o / 10 – 360o / 11) seems to be enough to disable a proton transfer between them.” The team made their measurements on ATP synthase motors from a species of thermophilic (heat-loving) bacteria. They feel they have found a coupling strategy in living systems that could demonstrate a general principle: “Here, we report the permissive nature of the coupling between proton transport and ATP synthesis of F0-F1, but such nature of the coupling can be general among other biological motor systems to connect critical well tuned microscopic events in the large domain motions.”1Mitome et al., “Thermophilic ATP synthase has a decamer c-ring: Indication of noninteger 10:3 H+/ATP ratio and permissive elastic coupling,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0403545101, published online 8/09/04.This discovery reveals a deeper level of design even more difficult to explain by evolution. (As expected, these authors make no reference to evolution in their paper.) A simple, easy-to-fathom machine would use the integer ratio; 3 protons yields one ATP. The 10:3 ratio, puzzling at first, actually shows superior engineering. It enables two disparate components with different operational requirements to be coupled together for the maximum efficiency of each. In software, it would be like the driver that allows a device to work with any operating system. In hardware, it would be like a tractor with a power-takeoff adapter that allows the engine to operate an attachment running at a different RPM. ATP synthase is made up of two finely tuned domains, F0 and F1, that operate under their own stringent requirements for function, but are useless without one another. Why would the F0 c-ring carousel evolve by itself, if it had no function vital to the cell? And how could the F1 system of six lobes, exquisitely-crafted for the synthesis of ATP, operate without an electrical motor to turn the camshaft? The camshaft itself is a perfectly-designed component, with just the right amount of elastic flexibility, to couple the two very different domains. Add to that the a subunit that feeds the protons at just the right rate and matches them to the appropriate active site on each c subunit, and the epsilon subunit that anchors the motor to the membrane, and you have an irreducibly complex system of irreducibly complex systems. The fact that this whole composite machine works at near 100% efficiency is proof of product, a contrivance that virtually shouts “made by intelligent design.”(Visited 42 times, 1 visits today)FacebookTwitterPinterestSave分享0